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Invitrogen™ pcDNA™4/HisMax A, B, & C Mammalian Expression Vectors
Designed to maximize protein expression in a variety of mammalian cells
Brand: Invitrogen™ V86420
Additional Details : Weight : 0.02000kg
Includes: The pcDNA4/HisMax A, B and C vectors, 20μg each, are supercoiled and provided lyophilized. 20μg of pcDNA4/HisMax/lacZ control plasmid is also provided. The pcDNA4/HisMax TOPO TA Expression Kit contains two boxes. The TOPO TA box contains 200ng of linearized and topoisomerase I-activated pcDNA4/HisMax-TOPO vector, dNTPs, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a lacZ expression control plasmid. The box contains transformation reagents including 21 x 50μL aliquots of TOP10 E. coli, S.O.C. medium, and a pUC19 supercoiled control plasmid.
Description
- Contains QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with promoter alone
- In addition to SP163-enhanced expression, pcDNA4/HisMax includes cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with Anti-Xpress Antibody
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Transfection | |
| Mammalian Expression Vector | |
The pcDNA™4/HisMax A, B, & C vectors, 20 μg each, are supercoiled and provided lyophilized. 20 μg of pcDNA™4/HisMax/lacZ control plasmid is also provided. Store vectors at -20°C. The pcDNA™4/HisMax TOPO™TA Expression Kit contains two boxes. The TOPO™ TA box contains 200 ng of linearized and topoisomerase I-activated pcDNA4/HisMax-TOPO™ vector, dNTPs, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a lacZ expression control plasmid. |
|
| His Tag (6x), Xpress Epitope Tag | |
| pcDNA | |
| Constitutive Expression |
| CMV | |
| Zeocin™ | |
| EK (Enterokinase) Recognition Site | |
| Restriction Enzyme/MCS | |
| pcDNA™ |
For Research Use Only. Not for use in diagnostic procedures.